Coding

Part:BBa_K4060020:Design

Designed by: Yan-Zhen, Chen   Group: iGEM21_NYCU-Taipei   (2021-09-30)


aprN (Nattokinase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 223
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 361


Design Notes

We use PCR technique to clone the gene fragment from genomic DNA of Bacillus subtilis natto. We added restriction enzyme cutting site, and ligated into pET-21a(+) to establish cloning vector.


Source

The source of the gene is from genomic DNA of Bacillus subtilis natto. Bacillus subtilis natto HS01 (a generous gift from Dr. Yi-Huang Hsueh)

References